survey frequency of verotoxigenic escherichia coli (vtec) in raw milk at kerman city

نویسندگان

mehdi hassanshahian

zahra shahi

چکیده

diarrhea is an important hygienic problem in under development countries. among pathogenic agents escherichia coli is the most etiologic agent of diarrhea in children below five years old. some e. coli strains produce cytotoxins that have cytopathic effect on vero cell and identified as verotoxin. this study performs for resolve frequency of vero toxigenic escherichia coli (vtec) in kerman city. samples include 375 raw milk sample that obtained from city and 122 raw milk samples that collected from cattle farms. vtec strains were serotype by entro pathogenic esherchia coli (epec) antiserum. verotoxin were measured by using vero cell. the results of this research show that from 375 raw milk samples (75.45 %) that prepared from kerman city 7 samples (1.85 %) were contaminated and from 122 samples (24.55 %) that prepared from cow growth two samples have contamination with vtec. neutralization experiment show that from 9 vtec strains that isolated from raw milk, 5 strain (55.5%) produce vt1 and 3 strains produce vt2 (33.3 %). also one strain (11.11 %) produces vt1 and vt2. degree of contamination within milks that sold in city than milk that prepare from cow growth have not any significant difference (p= 0.613). this study show vtec have very important in kerman city but o157:h7 serotype in the kerman city have low frequency.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Survey frequency of verotoxigenic Escherichia coli (VTEC) in raw milk at Kerman city

Diarrhea is an important hygienic problem in under development countries. Among pathogenic agents Escherichia coli is the most etiologic agent of diarrhea in children below five years old. Some E. coli strains produce cytotoxins that have cytopathic effect on vero cell and identified as verotoxin. This study performs for resolve frequency of Vero Toxigenic Escherichia coli...

متن کامل

Survey frequency of verotoxigenic Escherichia coli (VTEC) in raw milk at Kerman city

Diarrhea is an important hygienic problem in under development countries. Among pathogenic agents Escherichia coli is the most etiologic agent of diarrhea in children below five years old. Some E. coli strains produce cytotoxins that have cytopathic effect on vero cell and identified as verotoxin. This study performs for resolve frequency of Vero Toxigenic Escherichia coli...

متن کامل

Adherence Patterns of Verotoxigenic Escherichia coli (VTEC) Non-O157 Strains Isolated from Faecal Samples in Iran

The adherence property of 70 verotoxigenicE. coli (VTEC) strains, not belonging to O157: H7 serotype (non-O157) was checked on HeLa cells adherence assay. These strains were isolated from patients with diarrhea and healthy individuals. The results obtained in this study revealed that localised adherence (LA) was manifested by 3 strains of which two belonged to serogroups O111, O127 and the thir...

متن کامل

Detection of shiga-like toxigenic Escherichia coli from raw milk cheeses produced in Kerman-Iran

The Shiga-like toxin-producing escherichia coli (STEC) is the most important group of food-borne pathogens that emerged recently. These bacteria can cause severe health problems in humans like diarrhea, hemorrhagic colitis and hemolytic uraemic syndrome which have become a serious health problem in various countries. Cattle are thought to be a reservoir for e. coli STEC, and many foodborne dise...

متن کامل

Adherence Patterns of Verotoxigenic Escherichia coli (VTEC) Non-O157 Strains Isolated from Faecal Samples in Iran

The adherence property of 70 verotoxigenic E. coli (VTEC) strains, not belonging to O157: H7 serotype (non-O157) was checked on HeLa cells adherence assay. These strains were isolated from patients with diarrhea and healthy individuals. The results obtained in this study revealed that localised adherence (LA) was manifested by 3 strains of which two belonged to serogroups O111, O127 and the thi...

متن کامل

Agricultural, socioeconomic and environmental variables as risks for human verotoxigenic Escherichia coli (VTEC) infection in Finland

BACKGROUND Verotoxigenic E. coli (VTEC) is the cause of severe gastrointestinal infection especially among infants. Between 10 and 20 cases are reported annually to the National Infectious Disease Register (NIDR) in Finland. The aim of this study was to identify explanatory variables for VTEC infections reported to the NIDR in Finland between 1997 and 2006. We applied a hurdle model, applicable...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
international journal of advanced biological and biomedical research

ناشر: casrp publishing company

ISSN 2383-2762

دوره 2

شماره 1 2014

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023